《植物生理学报》 2016, 52(5): 653-658

黑果枸杞的叶片分化与快速繁殖

孙晓红^1,2,*, 位书磊^1,*, 宋强¹, 张玉刚², 戴洪义^2,**

青岛农业大学¹生命科学学院, 山东省植物生物技术重点实验室, ²园艺学院, 青岛市园艺植物遗传改良与育种重点实验室, 山东青岛266109

通信作者：孙晓红；E-mail: hydai@qau.edu.cn

摘　要：

以黑果枸杞(Lycium ruthenicum)成熟叶片为外植体, 诱导愈伤组织及不定芽的分化, 获得再生植株, 建立了黑果枸杞组织培养和快速繁殖技术新体系。结果表明: 诱导叶片脱分化形成愈伤组织的适宜培养基为MS+6-BA 1.0 mg•L^-1+NAA 0.2 mg•L^-1, 诱导率100%; 诱导愈伤组织分化和生长的适宜培养基为MS+ZT 1.4 mg•L^-1+NAA 0.02 mg•L^-1, 平均丛生芽数为23.7株; 诱导生根的适宜培养基为MS+IBA 0.6 mg•L^-1+NAA 0.4 mg•L^-1, 生根率100%; 将生长良好的组培苗移栽并覆盖保湿膜一周, 成活率达92.5%。试验成功建立了黑果枸杞快速繁殖体系。

关键词：黑果枸杞; 叶片分化; 组织培养; 植株再生

收稿：2016-04-08   修定：2016-05-03

资助：山东省良种产业化工程项目、“十二五”农村领域国家科技计划项目(2013BAD02B01)和青岛农业大学大学生科技创新项目。 * 共同第一作者。

Leaf differentiation and rapid propagation of Lycium ruthenicum

SUN Xiao-Hong^1,2,*, WEI Shu-Lei^1,*, SONG Qiang¹, ZHANG Yu-Gang², DAI Hong-Yi^2,**

¹Key Laboratory of Plant Biotechnology of Shandong Province, College of Life Sciences, ²Qingdao Key Laboratory of Genetic development and breeding in Horticultural Plants, College of Horticulture, Qingdao Agricultural University, Qingdao, Shandong 266109, China

Corresponding author: SUN Xiao-Hong; E-mail: hydai@qau.edu.cn

Abstract:

In the paper, we studied in vitro culture of Lycium ruthenicum by using leaf explants, including callus induction, adventitious shoot differentiation and plantlet regeneration. The results indicated that MS+6-BA 1.0 mg•L^-1+NAA 0.2 mg•L^-1 was the suitable medium for callus induction, with an induction rate of 100%; MS+ZT 1.4 mg•L^-1+NAA 0.02 mg•L^-1 was the optimizing medium for multiple shoot clumps, the multiplication coefficient could reach up to 23.7; MS+IBA 0.6 mg•L^-1+NAA 0.4 mg•L^-1 was the best medium for rooting, the rooting rate could reach to 100%. The strong rooting plantlets were transplanted into nutritive soil and covered the young plants with plastic farm film for a week, the survival rate were 92.5%. We have established a high-frequency regeneration system for L. ruthenicum.

Key words: Lycium ruthenicum; leaf differentiation; tissue culture; plantlet regeneration